The 2010 Varsity Cup clashes take place every Monday night between 1 February and 29 March. The fixtures for the 2010 competition are as follows:
| Fixture | Venue | Kick Off | Score | |
| Week One, Monday, February 1 | ||||
| Shimlas v UCT | Xerox NMMU Stadium | 2.30pm (TV) | ||
| NMMU v Maties | Xerox NMMU Stadium | 4.30pm (TV) | ||
| TUT v UJ | Tuks Rugby Stadium | 4.30pm (TV) | ||
| Tuks v Pukke | Tuks Rugby Stadium | 6.30pm (TV) | ||
| Week Two, Monday, February 8 | ||||
| UCT v Pukke | Groote Schuur Rugby Fields | 4.30pm (TV) | ||
| Maties v TUT | Danie Craven Stadium | 7.30pm | ||
| Shimlas v NMMU | Shimla Park | 6.30pm (TV) | ||
| Tuks v UJ | Tuks Rugby Stadium | 6.30pm | ||
| Week Three, Monday, February 15 | ||||
| TUT v Tuks | TUT Stadium | 4.30pm (TV) | ||
| UJ v Maties | UJ Stadium | 6.30pm | ||
| Pukke v Shimlas | Fanie du Toit Stadium | 6.30pm (TV) | ||
| NMMU v UCT | Xerox NMMU Stadium | 6.30pm | ||
| Week Four, Monday, February 22 | ||||
| UCT v TUT | Groote Schuur Rugby Fields | 4.30pm (TV) | ||
| Maties v Shimlas | Danie Craven Stadium | 7.30pm | ||
| Pukke v UJ | Fanie du Toit Stadium | 6.30pm | ||
| Tuks v NMMU | Tuks Rugby Stadium | 6.30pm (TV) | ||
| Week Five, Monday, March 1 | ||||
| Maties v Tuks | Danie Craven Stadium | 4.30pm (TV) | ||
| UJ v UCT | UJ Stadium | 6.30pm (TV) | ||
| NMMU v Pukke | Xerox NMMU Stadium | 6.30pm | ||
| Shimlas v TUT | Shimla Park | 6.30pm | ||
| Week Six, Monday, March 8 | ||||
| UJ v NMMU | UJ Stadium | 4.30pm (TV) | ||
| Tuks v Shimlas | Tuks Rugby Stadium | 6.30pm | ||
| Pukke v TUT | Fanie du Toit Stadium | 6.30pm | ||
| Maties v UCT | Danie Craven Stadium | 6.30pm (TV) | ||
| Week Seven, Monday, March 15 | ||||
| Pukke v Maties | Fanie du Toit Stadium | 4.30pm (TV) | ||
| UCT v Tuks | Groote Schuur Rugby Fields | 6.30pm | ||
| Shimlas v UJ | Shimla Park | 6.30pm (TV) | ||
| TUT v NMMU | TUT Stadium | 6.30pm | ||
| Semi-finals, Monday, March 22 | ||||
| Log 2 v Log 3 | TBC | 4.15pm (TV) | ||
| Log 1 v Log 4 | TBC | 6.30pm (TV) | ||
| Finals, Monday, March 29 | ||||
| Winner 1 v 4 v Winner 2 v 3 | TBC | 6.30pm (TV) | ||
Previous Varsity Cup Winners
2008 Stellenbosch (Maties) 16 – 10 UCT (Ikeys)
2009 Stellenbosch (Maties) 11 – 06 North-West University (Pukke)